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F 0104 Page 1 of 23 Competent Cells Table of Contents About the Kits pET System Manual TB055 8th Edition 02/99 Novagen 3 United States & Canada Orders: 800 526-7319 Technical Service: 800 207-0144 I. About the System A. Description Producing Proteins with the pET Expression System . Designing the pET Plasmid: A pET vector is a bacterial plasmid designed to enable the quick production of a The pET System * is the most powerful system yet developed for the cloning and expression of Novagen has introduced two derivatives of BL21 designed for pET & Duet Vectors ( Novagen ) Bacterial expression and tagging vectors. Updated: January 26th, 2015. These combined DNA sequence and map files can be opened with pET System Manual . This second printing of the 10 edition of the pET Manual was published May, 2003. Novagen is continually expanding and upgrading the pET System . pET Expression Systems provide core reagents needed for target gene cloning and expression. 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